LLP783_SpyCatcher-DNMT3A-CO
(Plasmid #211776)

• Purpose: SpyTag counterpart binding domain, SpyCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection
• Depositing Lab: Ryan Lister
• Publication: Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108. doi: 10.1093/nar/gkad1108.

Backbone

• Vector backbone: LLP252 pEF1a-NLS-scFvGCN4-DNMT3a
• Backbone manufacturer: Ryan Lister (Addgene plasmid # 100941)
• Vector type: Mammalian Expression
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Traptavudin-DNMT3A
• Species: H. sapiens (human)
• Mutation: Last 300 bp codon-optimised (CO) to detect DNMT3A expression distinct from endogenous mRNA
• Promoter: pEF1a and pSV40
• Tag / Fusion Protein:
  • 3xV5 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • LLP783_SpyCatcher-DNMT3A-CO.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.07.01.498378v1 for bioRxiv preprint.