LLP774_dCas9-Spy-Snoop-Sunx5-Avi-Tag-BFP (dCas9-SSSavi-BFP)
(Plasmid
#211767)
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PurposedCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), with BFP selection
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 211767 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePGK plasmid
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Vector typeMammalian Expression
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Selectable markersBFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namedCas9, Spy, Snoop, aGCN4, AviTags
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SpeciesH. sapiens (human)
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MutationdCas9 D10A and H840A
- Promoter pGK and pEF1a
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Tag
/ Fusion Protein
- 3xHA (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.07.01.498378v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LLP774_dCas9-Spy-Snoop-Sunx5-Avi-Tag-BFP (dCas9-SSSavi-BFP) was a gift from Ryan Lister (Addgene plasmid # 211767 ; http://n2t.net/addgene:211767 ; RRID:Addgene_211767) -
For your References section:
A modular dCas9-based recruitment platform for combinatorial epigenome editing. Swain T, Pflueger C, Freytag S, Poppe D, Pflueger J, Nguyen TV, Li JK, Lister R. Nucleic Acids Res. 2023 Nov 24:gkad1108. doi: 10.1093/nar/gkad1108. 10.1093/nar/gkad1108 PubMed 38000387
