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LLP773_pLenti-6xHRBKET-sgRNA
(Plasmid #211766)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211766 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti
  • Vector type
    Lentiviral
  • Selectable markers
    mCherry

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    gRNAs against six different human gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1)
  • gRNA/shRNA sequence
    PACC1-2: GGACCTACCGGCTCCGCG; EPCAM-2: GGGAGTTGGGGGAGTGAGT; B2M-4: GGGTAGGAGAGACTCACGC; KL-3: GGAGGCAGTCCCGGCTCGC; RBM3-1: GAAGACATCCGATCCAGCGG; HINT1-1: GACCAGGAACTTCCCCAAGC
  • Species
    H. sapiens (human)
  • Promoter pCMV and U6

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Also used GoldenGate cloning to insert multiple gRNA sequences.

Please visit https://www.biorxiv.org/content/10.1101/2022.07.01.498378v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LLP773_pLenti-6xHRBKET-sgRNA was a gift from Ryan Lister (Addgene plasmid # 211766 ; http://n2t.net/addgene:211766 ; RRID:Addgene_211766)

  • For your References section:

    A modular dCas9-based recruitment platform for combinatorial epigenome editing. Swain T, Pflueger C, Freytag S, Poppe D, Pflueger J, Nguyen TV, Li JK, Lister R. Nucleic Acids Res. 2023 Nov 24:gkad1108. doi: 10.1093/nar/gkad1108. 10.1093/nar/gkad1108 PubMed 38000387
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