pVG122
(Plasmid #211765)

• Purpose: Expresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.
• Depositing Lab: Sahand Jamal Rahi
• Publication: Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi: 10.1038/s41467-023-38959-8.

Backbone

• Vector backbone: pEZL105
• Total vector size (bp): 8000
• Vector type: Yeast Expression ; For single-copy integration (if cut with PmeI)
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: strongLOV
• Insert Size (bp): 636
• Mutation: compared to EL222, strongLOV contains Glu84Asp mutation
• Tag / Fusion Protein:
  • SV40 NLS and VP16 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Resource Information

• Supplemental Documents:
  • pVG122.seq

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry