pTwist GFP-Linker-VDAC1
(Plasmid
#211735)
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PurposeExpresses GFP tagged VDAC1 in mammalian cells. GFP is attached to the N-terminus of VDAC1 with a flexible linker.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 211735 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTwist
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Backbone manufacturerTwist Bioscience
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVoltage-dependent anion channel 1
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Insert Size (bp)1583
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Entrez GeneVDAC1 (a.k.a. PORIN, VDAC-1)
- Promoter CMV
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Tag
/ Fusion Protein
- GFP (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.09.08.556841 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTwist GFP-Linker-VDAC1 was a gift from Roland Malli (Addgene plasmid # 211735 ; http://n2t.net/addgene:211735 ; RRID:Addgene_211735) -
For your References section:
Visualizing interactions of VDAC1 in live cells using a tetracysteine tag. Pilic J, Oflaz FE, Gottschalk B, Erdogan YC, Graier WF, Malli R. bioRxiv 2023.09.08.556841 10.1101/2023.09.08.556841
Map uploaded by the depositor.
