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Addgene

pcDNA3.1 VDAC1-TC
(Plasmid #211733)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211733 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(-)
  • Backbone manufacturer
    gene universal
  • Backbone size w/o insert (bp) 5372
  • Total vector size (bp) 6254
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Voltage-dependent anion channel 1
  • Insert Size (bp)
    882
  • GenBank ID
  • Entrez Gene
    VDAC1 (a.k.a. PORIN, VDAC-1)
  • Promoter CMV
  • Tag / Fusion Protein
    • Tetracysteine (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.09.08.556841 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 VDAC1-TC was a gift from Roland Malli (Addgene plasmid # 211733 ; http://n2t.net/addgene:211733 ; RRID:Addgene_211733)

  • For your References section:

    Visualizing interactions of VDAC1 in live cells using a tetracysteine tag. Pilic J, Oflaz FE, Gottschalk B, Erdogan YC, Graier WF, Malli R. bioRxiv 2023.09.08.556841 10.1101/2023.09.08.556841
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