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Addgene

pUC19_CoV-2_linker
(Plasmid #211731)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211731 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2600
  • Total vector size (bp) 3734
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Linker fragment SARS-CoV-2
  • Species
    SARS-CoV-2; SV40; CMV
  • Insert Size (bp)
    1100

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19_CoV-2_linker was a gift from Thomas Klimkait (Addgene plasmid # 211731 ; http://n2t.net/addgene:211731 ; RRID:Addgene_211731)

  • For your References section:

    Rapid cloning-free mutagenesis of new SARS-CoV-2 variants using a novel reverse genetics platform. Kipfer ET, Hauser D, Lett MJ, Otte F, Urda L, Zhang Y, Lang CMR, Chami M, Mittelholzer C, Klimkait T. Elife. 2023 Nov 21;12:RP89035. doi: 10.7554/eLife.89035. 10.7554/eLife.89035 PubMed 37988285
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