CROPseq-iT7
(Plasmid
#211699)
-
Purpose(Empty Backbone) NIS-Seq-compatible lentiviral vector for expressing sgRNAs
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 211699 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backboneCROPseq
-
Backbone manufacturerChristoph Bock
- Backbone size (bp) 10237
-
Modifications to backboneInserted an inverted T7 promoter downstream of the sgRNA terminator
-
Vector typeMammalian Expression, Lentiviral, CRISPR
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.01.18.576210 for bioRxiv preprint.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
CROPseq-iT7 was a gift from Jonathan Schmid-Burgk (Addgene plasmid # 211699 ; http://n2t.net/addgene:211699 ; RRID:Addgene_211699) -
For your References section:
Cell Type-Agnostic Optical Perturbation Screening Using Nuclear In-Situ Sequencing (NIS-Seq). Fandrey CI, Konopka P, Jentzsch M, Zackria AA, Maasewerd S, Latz E, Schmid-Burgk JL. bioRxiv 2024.01.18.576210 10.1101/2024.01.18.576210
