pCF826-sgNT-1_U6-sgRNA-EFS-Cas9-P2A-mCherry2 Sequences (1)

Addgene Sequences:

Full (1)

Full Sequences from Addgene (1)

Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence).

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> Addgene NGS Result gtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcata gttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagct acaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttc gcgatgtacgggccagatattgacattgattattgactagttattaatagtaatcaattacggggtcatt agttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgccc aacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccatt gacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaag tacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgg gactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagt acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgg gagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaa atgggcggtaggcgtgtacggtgggaggtctatataagcagcgcgttttgcctgtactgggtctctctgg ttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagct tgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacc cttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacttgaaagcgaaagggaaaccag aggagctctctcgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggt gagtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaag cgggggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaa aacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcaga aggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagatcatta tataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttag acaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggccgctgatcttcagacctg gaggaggagatatgagggacaattggagaagtgaattatataaatataaagtagtaaaaattgaaccatt aggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagct ttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacggtacagg ccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattgaggcgcaacagca tctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtggaaagataccta aaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccactgctgtgccttgga atgctagttggagtaataaatctctggaacagatttggaatcacacgacctggatggagtgggacagaga aattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaa caagaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggt atataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgtactttctat agtgaatagagttaggcagggatattcaccattatcgtttcagacccacctcccaaccccgaggggaccc gacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagatccattcgattagtgaacg gatcggcactgcgtgcgccaattctgcagacaaatggcagtattcatccacaattttaaaagaaaagggg ggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacatacaaactaaagaatt acaaaaacaaattacaaaaattcaaaattttcgggtttattacagggacagcagagatccagtttggtta attaaggtaccgagggcctatttcccatgattccttcatatttgcatatacgatacaaggctgttagaga gataattagaattaatttgactgtaaacacaaagatattagtacaaaatacgtgacgtagaaagtaataa tttcttgggtagtttgcagttttaaaattatgttttaaaatggactatcatatgcttaccgtaacttgaa agtatttcgatttcttggctttatatatcttgtggaaaggacgaaacaccGGGCCAAACGTGCCCTGACG GgtttAagagctatgctggaaacagcatagcaagttTaaataaggctagtccgttatcaacttgaaaaag tggcaccgagtcggtgctttttttgaattcgctagctaggtcttgaaaggagtgggaattggctccggtg cccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgatc cggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttccc gagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccg ccagaacacaggaccggttctagagcgctgccaccatggacaagaagtacagcatcggcctggacatcgg caccaactctgtgggctgggccgtgatcaccgacgagtacaaggtgcccagcaagaaattcaaggtgctg ggcaacaccgaccggcacagcatcaagaagaacctgatcggagccctgctgttcgacagcggcgaaacag ccgaggccacccggctgaagagaaccgccagaagaagatacaccagacggaagaaccggatctgctatct gcaagagatcttcagcaacgagatggccaaggtggacgacagcttcttccacagactggaagagtccttc ctggtggaagaggataagaagcacgagcggcaccccatcttcggcaacatcgtggacgaggtggcctacc acgagaagtaccccaccatctaccacctgagaaagaaactggtggacagcaccgacaaggccgacctgcg gctgatctatctggccctggcccacatgatcaagttccggggccacttcctgatcgagggcgacctgaac cccgacaacagcgacgtggacaagctgttcatccagctggtgcagacctacaaccagctgttcgaggaaa accccatcaacgccagcggcgtggacgccaaggccatcctgtctgccagactgagcaagagcagacggct ggaaaatctgatcgcccagctgcccggcgagaagaagaatggcctgttcggaaacctgattgccctgagc ctgggcctgacccccaacttcaagagcaacttcgacctggccgaggatgccaaactgcagctgagcaagg acacctacgacgacgacctggacaacctgctggcccagatcggcgaccagtacgccgacctgtttctggc cgccaagaacctgtccgacgccatcctgctgagcgacatcctgagagtgaacaccgagatcaccaaggcc cccctgagcgcctctatgatcaagagatacgacgagcaccaccaggacctgaccctgctgaaagctctcg tgcggcagcagctgcctgagaagtacaaagagattttcttcgaccagagcaagaacggctacgccggcta cattgacggcggagccagccaggaagagttctacaagttcatcaagcccatcctggaaaagatggacggc accgaggaactgctcgtgaagctgaacagagaggacctgctgcggaagcagcggaccttcgacaacggca gcatcccccaccagatccacctgggagagctgcacgccattctgcggcggcaggaagatttttacccatt cctgaaggacaaccgggaaaagatcgagaagatcctgaccttccgcatcccctactacgtgggccctctg gccaggggaaacagcagattcgcctggatgaccagaaagagcgaggaaaccatcaccccctggaacttcg aggaagtggtggacaagggcgcttccgcccagagcttcatcgagcggatgaccaacttcgataagaacct gcccaacgagaaggtgctgcccaagcacagcctgctgtacgagtacttcaccgtgtataacgagctgacc aaagtgaaatacgtgaccgagggaatgagaaagcccgccttcctgagcggcgagcagaaaaaggccatcg tggacctgctgttcaagaccaaccggaaagtgaccgtgaagcagctgaaagaggactacttcaagaaaat cgagtgcttcgactccgtggaaatctccggcgtggaagatcggttcaacgcctccctgggcacataccac gatctgctgaaaattatcaaggacaaggacttcctggacaatgaggaaaacgaggacattctggaagata tcgtgctgaccctgacactgtttgaggacagagagatgatcgaggaacggctgaaaacctatgcccacct gttcgacgacaaagtgatgaagcagctgaagcggcggagatacaccggctggggcaggctgagccggaag ctgatcaacggcatccgggacaagcagtccggcaagacaatcctggatttcctgaagtccgacggcttcg ccaacagaaacttcatgcagctgatccacgacgacagcctgacctttaaagaggacatccagaaagccca ggtgtccggccagggcgatagcctgcacgagcacattgccaatctggccggcagccccgccattaagaag ggcatcctgcagacagtgaaggtggtggacgagctcgtgaaagtgatgggccggcacaagcccgagaaca tcgtgatcgaaatggccagagagaaccagaccacccagaagggacagaagaacagccgcgagagaatgaa gcggatcgaagagggcatcaaagagctgggcagccagatcctgaaagaacaccccgtggaaaacacccag ctgcagaacgagaagctgtacctgtactacctgcagaatgggcgggatatgtacgtggaccaggaactgg acatcaaccggctgtccgactacgatgtggaccatatcgtgcctcagagctttctgaaggacgactccat cgacaacaaggtgctgaccagaagcgacaagaaccggggcaagagcgacaacgtgccctccgaagaggtc gtgaagaagatgaagaactactggcggcagctgctgaacgccaagctgattacccagagaaagttcgaca atctgaccaaggccgagagaggcggcctgagcgaactggataaggccggcttcatcaagagacagctggt ggaaacccggcagatcacaaagcacgtggcacagatcctggactcccggatgaacactaagtacgacgag aatgacaagctgatccgggaagtgaaagtgatcaccctgaagtccaagctggtgtccgatttccggaagg atttccagttttacaaagtgcgcgagatcaacaactaccaccacgcccacgacgcctacctgaacgccgt cgtgggaaccgccctgatcaaaaagtaccctaagctggaaagcgagttcgtgtacggcgactacaaggtg tacgacgtgcggaagatgatcgccaagagcgagcaggaaatcggcaaggctaccgccaagtacttcttct acagcaacatcatgaactttttcaagaccgagattaccctggccaacggcgagatccggaagcggcctct gatcgagacaaacggcgaaaccggggagatcgtgtgggataagggccgggattttgccaccgtgcggaaa gtgctgagcatgccccaagtgaatatcgtgaaaaagaccgaggtgcagacaggcggcttcagcaaagagt ctatcctgcccaagaggaacagcgataagctgatcgccagaaagaaggactgggaccctaagaagtacgg cggcttcgacagccccaccgtggcctattctgtgctggtggtggccaaagtggaaaagggcaagtccaag aaactgaagagtgtgaaagagctgctggggatcaccatcatggaaagaagcagcttcgagaagaatccca tcgactttctggaagccaagggctacaaagaagtgaaaaaggacctgatcatcaagctgcctaagtactc cctgttcgagctggaaaacggccggaagagaatgctggcctctgccggcgaactgcagaagggaaacgaa ctggccctgccctccaaatatgtgaacttcctgtacctggccagccactatgagaagctgaagggctccc ccgaggataatgagcagaaacagctgtttgtggaacagcacaagcactacctggacgagatcatcgagca gatcagcgagttctccaagagagtgatcctggccgacgctaatctggacaaagtgctgtccgcctacaac aagcaccgggataagcccatcagagagcaggccgagaatatcatccacctgtttaccctgaccaatctgg gagcccctgccgccttcaagtactttgacaccaccatcgaccggaagaggtacaccagcaccaaagaggt gctggacgccaccctgatccaccagagcatcaccggcctgtacgagacacggatcgacctgtctcagctg ggaggcgacaagcgacctgccgccacaaagaaggctggacaggctaagaagaagaaagattacaaagacg atgacgataagggatccggcgcaacaaacttctctctgctgaaacaagccggagatgtcgaagagaatcc tggaccgATGGTCAGTAAAGGTGAAGAGGACAATATGGCTATCATCAAGGAGTTCATGCGCTTTAAGGTA CACATGGAAGGCTCAGTTAACGGGCATGAGTTCGAAATAGAGGGAGAAGGTGAAGGACGCCCCTATGAAG GCACCCAAACGGCTAAGCTGAAGGTAACTAAGGGCGGTCCCTTGCCCTTCGCCTGGGACATACTGAGCCC GCAGTTCATGTACGGTTCCAAAGCCTATGTAAAACACCCCGCCGACATTCCTGACTATCTTAAGCTGTCT TTTCCCGAAGGCTTTAACTGGGAAAGAGTAATGAATTTCGAGGATGGAGGGGTGGTCACTGTAACACAGG ATTCTAGTCTGCAAGATGGGGAGTTCATCTACAAGGTGAAGCTGCGCGGCACGAATTTTCCCTCAGATGG TCCTGTCATGCAGTGTCGGACTATGGGGTGGGAAGCAAGCACAGAAAGAATGTATCCTGAGGACGGGGCA CTGAAGGGAGAGATCAAACAGCGGCTCAAACTTAAAGACGGCGGGCACTATGACGCCGAAGTTAAGACAA CTTACAAGGCCAAGAAGCCCGTTCAGTTGCCGGGCGCATACAACGTGGACATAAAGCTCGACATCTTGTC CCATAATGAGGACTACACAATCGTGGAACAGTATGAACGGGCCGAGGGCAGACATTCTACCGGGGGGATG GATGAGCTTTACAAGTAAacgcgttaagtcgacaatcaacctctggattacaaaatttgtgaaagattga ctggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgc tattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggag ttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggg gcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaact catcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttg tcggggaaatcatcgtcctttccttggctgctcgcctgtgttgccacctggattctgcgcgggacgtcct tctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcc tcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcgtcgactt taagaccaatgacttacaaggcagctgtagatcttagccactttttaaaagaaaaggggggactggaagg gctaattcactcccaacgaagacaagatctgctttttgcttgtactgggtctctctggttagaccagatc tgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgc ttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagt gtggaaaatctctagcagggcccgtttaaacccgctgatcagcctcgactgtgccttctagttgccagcc atctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaa taaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcagg acagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggcttctga ggcggaaagaaccagccattaatgaaaagaaccagccattaatgaatcggccaacgcgcggggagaggcg gtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcg agcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaac atgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggc tccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactata aagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccgga tacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagtt cggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgcctt atccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggt aacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggct acactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtag ctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgc agaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaact cacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatg aagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgag gcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataacta cgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctcc agatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcc tccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacg ttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttc ccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccg atcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctctta ctgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtg tatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaacttta aaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatcca gttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtg agcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcata ctcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaat gtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgac

pCF826-sgNT-1_U6-sgRNA-EFS-Cas9-P2A-mCherry2 Sequences (1)

Full Sequences from Addgene (1)

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