pLenti-h.Rhno1 T52A (B)-C-Myc-DDK-P2A-Puro
(Plasmid
#211621)
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PurposeRhno1 T52A
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 211621 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti
- Backbone size w/o insert (bp) 7055
- Total vector size (bp) 7769
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehRHNO1
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SpeciesH. sapiens (human)
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Insert Size (bp)714
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MutationT52A
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Entrez GeneRHNO1 (a.k.a. C12orf32, HKMT1188, RHINO)
- Promoter CMV
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Tag
/ Fusion Protein
- myc-flag (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti-h.Rhno1 T52A (B)-C-Myc-DDK-P2A-Puro was a gift from Agnel Sfeir (Addgene plasmid # 211621 ; http://n2t.net/addgene:211621 ; RRID:Addgene_211621) -
For your References section:
RHINO directs MMEJ to repair DNA breaks in mitosis. Brambati A, Sacco O, Porcella S, Heyza J, Kareh M, Schmidt JC, Sfeir A. Science. 2023 Aug 11;381(6658):653-660. doi: 10.1126/science.adh3694. Epub 2023 Jul 13. 10.1126/science.adh3694 PubMed 37440612