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PolyA Barcode Library
(Pooled Library #211610)

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  • Purpose

    This retroviral plasmid barcode library is sufficient to label more than 30,000 cells with a barcode collision rate less than 1%. The 3' untranslated region of the EGFP coding sequence contains a 32-bp semi-random barcode before the polyA sequence. Single-cell split barcoding (SISBAR) allows simultaneous capture of the transcriptome and viral barcode sequences to infer cellular identities and lineage information across developmental stages.

  • Vector Backbone

    pDest-EF1a-EGFP-bGHPA(reverse) - Sequence (GBK, 17 KB)

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 211610 pDest-EF1α-EGFP-32bp Barcode PolyA Library 1 $640 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Number of barcodes
    >3 million
  • Virus type
    Retrovirus

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼15 µL
  • Concentration
    100 ng/µL

Resource Information

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PolyA Barcoding Library was a gift from Yuejun Chen (Addgene #211610)

  • For your References section:

    Mapping of clonal lineages across developmental stages in human neural differentiation. You Z, Wang L, He H, Wu Z, Zhang X, Xue S, Xu P, Hong Y, Xiong M, Wei W, Chen Y. Cell Stem Cell. 2023 Apr 6;30(4):473-487.e9. doi: 10.1016/j.stem.2023.02.007. Epub 2023 Mar 17. PubMed 36933556 (Link opens in a new window)
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