pCMV-PEmax-SpG-P2A-hMLH1dn
(Plasmid
#211550)
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PurposePrime editing in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 211550 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePEmax-SpG-P2A-hMLH1dn
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SpeciesSynthetic; Cas9 is from S. pyogenes; M-MLV RT is from the Moloney murine leukemia virus
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7-forward
- 3′ sequencing primer BGH-reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-PEmax-SpG-P2A-hMLH1dn was a gift from Yongsub Kim (Addgene plasmid # 211550 ; http://n2t.net/addgene:211550 ; RRID:Addgene_211550) -
For your References section:
CRISPR prime editing for unconstrained correction of oncogenic KRAS variants. Jang G, Kweon J, Kim Y. Commun Biol. 2023 Jun 30;6(1):681. doi: 10.1038/s42003-023-05052-1. 10.1038/s42003-023-05052-1 PubMed 37391511
