pcDNA3-HA-Sumo1
(Plasmid #21154)

• Purpose: Mammalian expression of human Sumo1 with HA tag
• Depositing Lab: Junying Yuan
• Publication: Terui et al J Biol Chem. 2004 Jul 2. 279(27):28257-65.

Backbone

• Vector backbone: pcDNA3.1 HA
• Modifications to backbone: BGH polyA terminator removed during cloning of Sumo1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pcDNA3-HA-Sumo1
• Alt name: Sumo1
• Species: H. sapiens (human)
• Entrez Gene: SUMO1 (a.k.a. DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: SmaI (destroyed during cloning)
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid does not contain the BGH polyA terminator present in the standard pcDNA3 backbone. It is not known whether the removal of the terminator affects expression.