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Addgene

hLig4 guide2 in pX458
(Plasmid #211536)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211536 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pX458
  • Backbone size w/o insert (bp) 9272
  • Total vector size (bp) 9292
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    eGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    sgRNA hLig4
  • gRNA/shRNA sequence
    TTGCTTAATTTACCTAGAGA
  • Species
    Synthetic
  • Entrez Gene
    LIG4 (a.k.a. LIG4S)
  • Promoter u6

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hLig4 guide2 in pX458 was a gift from Agnel Sfeir (Addgene plasmid # 211536 ; http://n2t.net/addgene:211536 ; RRID:Addgene_211536)

  • For your References section:

    RHINO directs MMEJ to repair DNA breaks in mitosis. Brambati A, Sacco O, Porcella S, Heyza J, Kareh M, Schmidt JC, Sfeir A. Science. 2023 Aug 11;381(6658):653-660. doi: 10.1126/science.adh3694. Epub 2023 Jul 13. 10.1126/science.adh3694 PubMed 37440612
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