pMXS-IRES-Blast coUGP2
(Plasmid
#211531)
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PurposeExpresses codon optimized UGP2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 211531 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMXS-IRES-BLAST
- Backbone size w/o insert (bp) 5623
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Vector typeMammalian Expression, Retroviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUGP2
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SpeciesH. sapiens (human)
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Insert Size (bp)1530
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GenBank ID7360
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Entrez GeneUGP2 (a.k.a. UDPG, UGPP2, UDPGP2, pHC379)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer AGTAGACGGCATCGCAGCTTGGATA
- 3′ sequencing primer GGC GGA ATT TAC GTA GCG GCC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMXS-IRES-Blast coUGP2 was a gift from Richard Possemato (Addgene plasmid # 211531 ; http://n2t.net/addgene:211531 ; RRID:Addgene_211531)