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pGL4.R_TRE_minCMV
(Plasmid #211518)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211518 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL4.33[luc2P/SRE/Hygro]
  • Backbone manufacturer
    Promega
  • Modifications to backbone
    Hygromycin CDS was replaced with Renilla luciferase CDS. Then the minCMV promoter was ligated into the backbone following ApaI and KpnI digestion. Finally, annealed oligos containing restriction enzyme sites were ligated following KpnI digestion.
  • Vector type
    Bacterial Expression
  • Promoter SV40 and minCMV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL4.R_TRE_minCMV was a gift from Justin English (Addgene plasmid # 211518 ; http://n2t.net/addgene:211518 ; RRID:Addgene_211518)

  • For your References section:

    Discovery and Validation of Context-Dependent Synthetic Mammalian Promoters. Zahm AM, Owens WS, Himes SR, Rondem KE, Fallon BS, Gormick AN, Bloom JS, Kosuri S, Chan H, English JG. bioRxiv. 2023 May 11:2023.05.11.539703. doi: 10.1101/2023.05.11.539703. Preprint. 10.1101/2023.05.11.539703 PubMed 37214829
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