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Addgene

6xHis-SNAP-PI(3,4,5)P3 Probe
(Plasmid #211514)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211514 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-28 a (+)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    BTK1-PH
  • Alt name
    The biosensor for PI(3,4,5)P3 is the PH domain from BTK (Fukuda et al., 1996; Salim et al., 1996)
  • Species
    H. sapiens (human)
  • Tag / Fusion Protein
    • 6xhis-SNAP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The biosensor for PI(3,4,5)P3 is the PH domain from BTK (Fukuda et al., 1996; Salim et al., 1996).

Please visit https://www.biorxiv.org/content/10.1101/2023.10.18.562882v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    6xHis-SNAP-PI(3,4,5)P3 Probe was a gift from Hannes Maib & David Murray (Addgene plasmid # 211514 ; http://n2t.net/addgene:211514 ; RRID:Addgene_211514)

  • For your References section:

    Recombinant biosensors for multiplex and super-resolution imaging of phosphoinositides. Maib H, Adarska P, Hunton R, Vines JH, Strutt D, Bottanelli F, Murray DH. J Cell Biol. 2024 Jun 3;223(6):e202310095. doi: 10.1083/jcb.202310095. Epub 2024 Apr 5. 10.1083/jcb.202310095 PubMed 38578646
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