6xHis-SNAP-PI(3,4)P2 Probe
(Plasmid #211511)

• Purpose: For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4-Bisphosphate (PI(3,4)P2) from bacteria
• Depositing Labs: Hannes Maib, David Murray
• Publication: Maib et al J Cell Biol. 2024 Jun 3;223(6):e202310095. doi: 10.1083/jcb.202310095. Epub 2024 Apr 5.

Backbone

• Vector backbone: pET-28 a (+)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: TAPP1 cPHx3
• Alt name: The biosensor for PI(3,4)P2 is the triple repeat of the C-terminal PH domain from TAPP1 (Goulden et al., 2019)
• Species: H. sapiens (human)
• Tag / Fusion Protein:
  • 6xhis-SNAP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The biosensor for PI(3,4)P2 is the triple repeat of the C-terminal PH domain from TAPP1 (Goulden et al., 2019).

Please visit https://www.biorxiv.org/content/10.1101/2023.10.18.562882v1 for bioRxiv preprint.

Please note: This plasmid contains a deletion removing the six C-terminal amino acids from the 6xHis-SNAP-PI(3,4)P2 Probe insert. This deletion is not known to affect plasmid function.