6xHis-SNAP-PI(4)P Probe
(Plasmid
#211509)
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PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-4-Phosphate (PI(4)P) from bacteria
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 211509 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET-28 a (+)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSidC (P4C)
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Alt nameThe biosensor for PI(4)P is the is the effector domain of SidC (P4C) (Dolinsky et al., 2014)
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SpeciesLegionella pneumophila
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Tag
/ Fusion Protein
- 6xhis-SNAP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The biosensor for PI(4)P is the is the effector domain of SidC (P4C) (Dolinsky et al., 2014).
Please visit https://www.biorxiv.org/content/10.1101/2023.10.18.562882v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
6xHis-SNAP-PI(4)P Probe was a gift from Hannes Maib & David Murray (Addgene plasmid # 211509 ; http://n2t.net/addgene:211509 ; RRID:Addgene_211509) -
For your References section:
Recombinant biosensors for multiplex and super-resolution imaging of phosphoinositides. Maib H, Adarska P, Hunton R, Vines JH, Strutt D, Bottanelli F, Murray DH. J Cell Biol. 2024 Jun 3;223(6):e202310095. doi: 10.1083/jcb.202310095. Epub 2024 Apr 5. 10.1083/jcb.202310095 PubMed 38578646