6xHis-SNAP-PI(3)P Probe
(Plasmid #211508)

• Purpose: For expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteria
• Depositing Labs: Hannes Maib, David Murray
• Publication: Maib et al J Cell Biol. 2024 Jun 3;223(6):e202310095. doi: 10.1083/jcb.202310095. Epub 2024 Apr 5.

Backbone

• Vector backbone: pET-28 a (+)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: FYVE-Hrs x 2
• Alt name: The biosensor for PI(3)P is the tandem repeat of the FYVE domain from HRS (Burd and Emr, 1998)
• Species: H. sapiens (human)
• Entrez Gene: PIKFYVE (a.k.a. CFD, FAB1, HEL37, PIP5K, PIP5K3, ZFYVE29)
• Tag / Fusion Protein:
  • 6xhis-SNAP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The biosensor for PI(3)P is the tandem repeat of the FYVE domain from HRS (Burd and Emr, 1998).

Please visit https://www.biorxiv.org/content/10.1101/2023.10.18.562882v1 for bioRxiv preprint.