pAS133
(Plasmid
#211504)
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PurposeAAV genome to express mTagBF2 under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 211504 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
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Vector typeMammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemTagBF2
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SpeciesH. sapiens (human), M. musculus (mouse)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAS133 was a gift from Randall Platt (Addgene plasmid # 211504 ; http://n2t.net/addgene:211504 ; RRID:Addgene_211504) -
For your References section:
Transcriptional linkage analysis with in vivo AAV-Perturb-seq. Santinha AJ, Klingler E, Kuhn M, Farouni R, Lagler S, Kalamakis G, Lischetti U, Jabaudon D, Platt RJ. Nature. 2023 Oct;622(7982):367-375. doi: 10.1038/s41586-023-06570-y. Epub 2023 Sep 20. 10.1038/s41586-023-06570-y PubMed 37730998