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Addgene

gRNA-10Xcapture-PuroR
(Plasmid #211496)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211496 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Lenti
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR, Synthetic Biology
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    U6-gRNA
  • Alt name
    gRNA for SAM CRISPRa with 10X capture sequence 2
  • Species
    Synthetic
  • Promoter U6

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (unknown if destroyed)
  • 3′ cloning site BbsI (unknown if destroyed)
  • 5′ sequencing primer NA
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Ef1a-PuroR
  • Alt name
    Puro resistance gene for the selection of integration events
  • Species
    Synthetic
  • Promoter EF1a

Cloning Information for Gene/Insert 2

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The capture sequence has been inserted into the Addgene plasmid #73797

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    gRNA-10Xcapture-PuroR was a gift from Antonella Fidanza & Lesley Forrester (Addgene plasmid # 211496 ; http://n2t.net/addgene:211496 ; RRID:Addgene_211496)

  • For your References section:

    A novel human pluripotent stem cell gene activation system identifies IGFBP2 as a mediator in the production of haematopoietic progenitors in vitro. Petazzi P, Ventura T, Luongo FP, McClafferty H, May A, Taylor HA, Shipston MJ, Romano N, Forrester LM, Menendez P, Fidanza A. eLife. 2024 Dec 23;13:RP94884. doi: 10.7554/eLife.94884. 10.7554/eLife.94884 PubMed 39714446
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