Skip to main content
Addgene

pCMV-tBID
(Plasmid #21149)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21149 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCMV-5a
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pCMV-tBID
  • Alt name
    pHL174
  • Species
    M. musculus (mouse)
  • GenBank ID
    AAC71064.1 (Truncated: AA60-195)
  • Tag / Fusion Protein
    • flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-tBID was a gift from Junying Yuan (Addgene plasmid # 21149 ; http://n2t.net/addgene:21149 ; RRID:Addgene_21149)

  • For your References section:

    Cleavage of BID by caspase 8 mediates the mitochondrial damage in the Fas pathway of apoptosis. Li H, Zhu H, Xu CJ, Yuan J. Cell. 1998 Aug 21. 94(4):491-501. 10.1016/S0092-8674(00)81590-1 PubMed 9727492
Related items:
Commonly requested with: