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Addgene

GFP-LKB1-KM
(Plasmid #21148)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21148 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    GFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GFP-LKB1-KM
  • Alt name
    GFP-KMPJ
  • Alt name
    Stk11
  • Species
    M. musculus (mouse)
  • Mutation
    K78M
  • Entrez Gene
    Stk11 (a.k.a. Lkb1, Par-4)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR 1 (unknown if destroyed)
  • 3′ cloning site Sal 1 (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-LKB1-KM was a gift from Junying Yuan (Addgene plasmid # 21148 ; http://n2t.net/addgene:21148 ; RRID:Addgene_21148)

  • For your References section:

    The Peutz-Jegher gene product LKB1 is a mediator of p53-dependent cell death. Karuman P, Gozani O, Odze RD, Zhou XC, Zhu H, Shaw R, Brien TP, Bozzuto CD, Ooi D, Cantley LC, Yuan J. Mol Cell. 2001 Jun . 7(6):1307-19. 10.1016/S1097-2765(01)00258-1 PubMed 11430832
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