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pLenti_Cas9_T2A_mNeonGreen_P2A_blasticidin
(Plasmid #211471)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211471 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLentiCas9-T2A-GFP
  • Backbone manufacturer
    Addgene 78548
  • Total vector size (bp) 13724
  • Modifications to backbone
    Replaced GFP with mNeonGreen
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cas9_T2A_mNeonGreen_P2A_BlasticidinR
  • Species
    Synthetic
  • Insert Size (bp)
    5426
  • Promoter hU6, EF-1a
  • Tag / Fusion Protein
    • 3xFlag

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer agcagagagaagtttgttgc
  • 3′ sequencing primer gggcagagcgcacatcgccc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.04.15.537042 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti_Cas9_T2A_mNeonGreen_P2A_blasticidin was a gift from James Eshleman (Addgene plasmid # 211471 ; http://n2t.net/addgene:211471 ; RRID:Addgene_211471)

  • For your References section:

    CRISPR-Cas9 for selective targeting of somatic mutations in pancreatic cancers. Teh SSK, Bowland K, Halper-Stromberg E, Kotwal A, Bennett A, Skaist A, Tang J, Cai F, Macoretta A, Liang H, Kamiyama H, Wheelan S, Lin MT, Hruban RH, Hung CF, Goldstein M, Scharpf RB, Roberts NJ, Eshleman JR. NAR Cancer. 2024 Jun 19;6(2):zcae028. doi: 10.1093/narcan/zcae028. eCollection 2024 Jun. 10.1093/narcan/zcae028 PubMed 38915758
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