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Addgene

PCMV-flag-Caspase11
(Plasmid #21145)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21145 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-3flag 7.1
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Caspase11
  • Alt name
    Casp11
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1122
  • Entrez Gene
    Casp4 (a.k.a. CASP-11, CASP-4, Casp11, Caspl, ich-3)
  • Tag / Fusion Protein
    • flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PCMV-flag-Caspase11 was a gift from Junying Yuan (Addgene plasmid # 21145 ; http://n2t.net/addgene:21145 ; RRID:Addgene_21145)

  • For your References section:

    Caspase-11 regulates cell migration by promoting Aip1-Cofilin-mediated actin depolymerization. Li J, Brieher WM, Scimone ML, Kang SJ, Zhu H, Yin H, von Andrian UH, Mitchison T, Yuan J. Nat Cell Biol. 2007 Mar . 9(3):276-86. 10.1038/ncb1541 PubMed 17293856
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