pcDNA3-BG4-NLS-mCer
(Plasmid
#211448)
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Purposemammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequence
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Depositing Lab
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Publication
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 211448 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3-mCer
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAnti-DNA G-quadruplex single chain antibody BG4
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SpeciesSynthetic
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Tag
/ Fusion Protein
- NLS-mCerulean (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
Resource Information
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A portion of this plasmid was derived from a plasmid made byShankar Balasubramanian, plasmid pSANG10-3F-BG4 (Addgene #55756)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
generated by Julia Kutz (Leibniz Institute on Aging - Fritz Lipmann Institute, D-07745 Jena, Germany)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-BG4-NLS-mCer was a gift from Helmut Pospiech (Addgene plasmid # 211448 ; http://n2t.net/addgene:211448 ; RRID:Addgene_211448)
