pcDNA3-C17orf53_1-87-mVen
(Plasmid
#211442)
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Purposemammalian expression of the N-terminal amino acids 1-87 of human C17orf53 C-terminally fused to mVenus
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 211442 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3-mVen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameC17orf53
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Alt nameHROB
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Alt nameMCM8IP
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SpeciesH. sapiens (human)
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MutationDeletion of aa 88-646
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Entrez GeneHROB (a.k.a. C17orf53, MCM8IP)
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Tag
/ Fusion Protein
- mVenus (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
generated by Sheikh Anika Rahman (Leibniz Institute on Aging - Fritz Lipmann Institute, D-07745 Jena, Germany)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-C17orf53_1-87-mVen was a gift from Helmut Pospiech (Addgene plasmid # 211442 ; http://n2t.net/addgene:211442 ; RRID:Addgene_211442)