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pFastBac1-6His-DHX9
(Plasmid #211440)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211440 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac1
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DHX9
  • Alt name
    NDH2
  • Species
    H. sapiens (human)
  • Entrez Gene
    DHX9 (a.k.a. DDX9, LKP, NDH2, NDHII, RHA)
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

generated by Suisheng Zhang (Leibniz Institute on Aging - Fritz Lipmann Institute, D-07745 Jena, Germany)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac1-6His-DHX9 was a gift from Frank Grosse & Helmut Pospiech (Addgene plasmid # 211440 ; http://n2t.net/addgene:211440 ; RRID:Addgene_211440)

  • For your References section:

    Domain structure of human nuclear DNA helicase II (RNA helicase A). Zhang S, Grosse F. J Biol Chem. 1997 Apr 25. 272(17):11487-94. 10.1074/jbc.272.17.11487 PubMed 9111062
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