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Addgene

pBabePuro-bcl2
(Plasmid #21144)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21144 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBabePuro
  • Backbone size w/o insert (bp) 5035
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pBabePuro-bcl 2
  • Alt name
    pJ436
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1215

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabePuro-bcl2 was a gift from Junying Yuan (Addgene plasmid # 21144 ; http://n2t.net/addgene:21144 ; RRID:Addgene_21144)

  • For your References section:

    Induction of apoptosis in fibroblasts by IL-1 beta-converting enzyme, a mammalian homolog of the C. elegans cell death gene ced-3. Miura M, Zhu H, Rotello R, Hartwieg EA, Yuan J. Cell. 1993 Nov 19. 75(4):653-60. 10.1016/0092-8674(93)90486-A PubMed 8242741
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