pPAL7-C17orf53_1-241
(Plasmid
#211437)
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Purposebacterial expression of the N-terminal amino acids 1-241 of human C17orf53 N-terminally fused to Profinity eXact-Tag
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 211437 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepPAL7
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Backbone manufacturerBio-Rad
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameC17orf53
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Alt nameHROB
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Alt nameMCM8IP
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SpeciesH. sapiens (human)
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MutationDeletion of aa 242-646
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Entrez GeneHROB (a.k.a. C17orf53, MCM8IP)
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Tag
/ Fusion Protein
- Profinity eXact-Tag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
generated by Julia Kutz (Leibniz Institute on Aging - Fritz Lipmann Institute, D-07745 Jena, Germany)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPAL7-C17orf53_1-241 was a gift from Helmut Pospiech (Addgene plasmid # 211437 ; http://n2t.net/addgene:211437 ; RRID:Addgene_211437)