pRSF-DUET-C17orf53_1-87-Strep
(Plasmid
#211433)
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Purposebacterial expression of the N-terminal amino acids 1-87 of human C17orf53 C-terminally fused to a Strep-Tag
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 211433 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRSFDuet-1
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameC17orf53
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Alt nameHROB
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Alt nameMCM8IP
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SpeciesH. sapiens (human)
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MutationDeletion of aa 88-646
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Entrez GeneHROB (a.k.a. C17orf53, MCM8IP)
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Tag
/ Fusion Protein
- Strep-Tag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site NdeI (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
generated by Julia Kutz (Leibniz Institute on Aging - Fritz Lipmann Institute, D-07745 Jena, Germany)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSF-DUET-C17orf53_1-87-Strep was a gift from Helmut Pospiech (Addgene plasmid # 211433 ; http://n2t.net/addgene:211433 ; RRID:Addgene_211433)