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pRPA70-IRES-Ven-RPA32-IRES-RPA14
(Plasmid #211430)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211430 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmVenus-C1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RPA70 - IRES - RPA32 - IRES - RPA14
  • Alt name
    RPA1 and RPA2 and RPA3
  • Species
    H. sapiens (human)
  • Entrez Gene
    RPA1 (a.k.a. HSSB, MST075, PFBMFT6, REPA1, RF-A, RP-A, RPA70)
  • Entrez Gene
    RPA2 (a.k.a. REPA2, RP-A p32, RP-A p34, RPA32)
  • Entrez Gene
    RPA3 (a.k.a. REPA3, RP-A p14)
  • Tag / Fusion Protein
    • mVenus (on RPA32) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (destroyed during cloning)
  • 3′ cloning site XbaI (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

generated by Julia Kutz (Leibniz Institute on Aging - Fritz Lipmann Institute, D-07745 Jena, Germany)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRPA70-IRES-Ven-RPA32-IRES-RPA14 was a gift from Helmut Pospiech (Addgene plasmid # 211430 ; http://n2t.net/addgene:211430 ; RRID:Addgene_211430)
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