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pcDNA4/TO-Cdc45HA
(Plasmid #211427)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211427 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4/TO
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cdc45
  • Species
    H. sapiens (human)
  • Entrez Gene
    CDC45 (a.k.a. CDC45L, CDC45L2, MGORS7, PORC-PI-1)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

generated by Dennis Keller-Koalick (Leibniz Institute on Aging - Fritz Lipmann Institute, D-07745 Jena, Germany)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4/TO-Cdc45HA was a gift from Helmut Pospiech (Addgene plasmid # 211427 ; http://n2t.net/addgene:211427 ; RRID:Addgene_211427)

  • For your References section:

    Cdc45 is limiting for replication initiation in humans. Kohler C, Koalick D, Fabricius A, Parplys AC, Borgmann K, Pospiech H, Grosse F. Cell Cycle. 2016;15(7):974-85. doi: 10.1080/15384101.2016.1152424. 10.1080/15384101.2016.1152424 PubMed 26919204
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