pEGFP-N1-Cdc45
(Plasmid
#211426)
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Purposemammalian expression of human Cdc45 C-terminally fused to EGFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 211426 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCdc45
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SpeciesH. sapiens (human)
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Entrez GeneCDC45 (a.k.a. CDC45L, CDC45L2, MGORS7, PORC-PI-1)
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
generated by Carsten Koehler (Leibniz Institute on Aging - Fritz Lipmann Institute, D-07745 Jena, Germany)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-N1-Cdc45 was a gift from Helmut Pospiech (Addgene plasmid # 211426 ; http://n2t.net/addgene:211426 ; RRID:Addgene_211426) -
For your References section:
Cdc45 is limiting for replication initiation in humans. Kohler C, Koalick D, Fabricius A, Parplys AC, Borgmann K, Pospiech H, Grosse F. Cell Cycle. 2016;15(7):974-85. doi: 10.1080/15384101.2016.1152424. 10.1080/15384101.2016.1152424 PubMed 26919204