pCMV-Caspase1-flag
(Plasmid #21142)

• Depositing Lab: Junying Yuan
• Publication: Li et al J Cell Biol. 2008 Apr 21. 181(2):321-33.

Backbone

• Vector backbone: pCMV
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: caspase-1
• Alt name: pS34
• Alt name: pCMV-Casp1-flag
• Species: M. musculus (mouse)
• GenBank ID: BC008152
• Entrez Gene: Casp1 (a.k.a. ICE, Il1bc)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sal I (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hgh-pA-R

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Compared to GenBank reference sequence BC008152, the caspase-1 insert in this plasmid contains a D56N mutation.