pRSFDuet-1-Cdc45-6His
(Plasmid
#211379)
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Purposebacterial expression of human Cdc45 C-terminally fused to 6xHis-Tag
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 211379 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRSFDuet-1
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCdc45
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SpeciesH. sapiens (human)
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Entrez GeneCDC45 (a.k.a. CDC45L, CDC45L2, MGORS7, PORC-PI-1)
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Tag
/ Fusion Protein
- 6xHis (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (destroyed during cloning)
- 3′ cloning site XhoI (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
generated by Christian Usskilat (Leibniz Institute on Aging - Fritz Lipmann Institute, D-07745 Jena, Germany)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSFDuet-1-Cdc45-6His was a gift from Helmut Pospiech (Addgene plasmid # 211379 ; http://n2t.net/addgene:211379 ; RRID:Addgene_211379) -
For your References section:
DNA binding properties of human Cdc45 suggest a function as molecular wedge for DNA unwinding. Szambowska A, Tessmer I, Kursula P, Usskilat C, Prus P, Pospiech H, Grosse F. Nucleic Acids Res. 2014 Feb;42(4):2308-19. doi: 10.1093/nar/gkt1217. Epub 2013 Nov 28. 10.1093/nar/gkt1217 PubMed 24293646
