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PurposeDnmt1 targeting epegRNA with COM insertion in gRNA scaffold for v3b PE-eVLP production
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 211376 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBR322
- Backbone size w/o insert (bp) 2165
- Total vector size (bp) 2373
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCOM-epegRNA
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Alt nameDnmt1-locus targeting epegRNA encoding 4-bp substitution. COM RNA hairpin insertion in gRNA scaffold.
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Insert Size (bp)208
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
COM-epegRNA-Dnmt1 was a gift from David Liu (Addgene plasmid # 211376 ; http://n2t.net/addgene:211376 ; RRID:Addgene_211376) -
For your References section:
Engineered virus-like particles for transient delivery of prime editor ribonucleoprotein complexes in vivo. An M, Raguram A, Du SW, Banskota S, Davis JR, Newby GA, Chen PZ, Palczewski K, Liu DR. Nat Biotechnol. 2024 Jan 8. doi: 10.1038/s41587-023-02078-y. 10.1038/s41587-023-02078-y PubMed 38191664