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PB-mCherry-DMDEx23-eGFP
(Plasmid #211366)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211366 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PB-CAG-GFPd2 (Addgene #115665)
  • Backbone size w/o insert (bp) 5592
  • Total vector size (bp) 7632
  • Modifications to backbone
    Cutting-out of GFPd2 (XmaI, NotI)
  • Vector type
    Mammalian Expression
  • Selectable markers
    eGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry-DMDEx23-eGFP
  • Species
    Discosoma sp. (mCherry), Aequorea victoria (eGFP)
  • Insert Size (bp)
    2040
  • Mutation
    mCherry interrupted by mdx dystrophin exon 23 between nucleotide 105 and 106 flanked by intronic sequences. Donor splice site downstream of mdx exon 23 is based on the physiological donor splice site sequence of murine DMD intron 23.
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XmaI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer ATCCCGGGGACTCAGATCTCGAGGCCACCATG
  • 3′ sequencing primer TCGCGGCCGCTTTACTTGTACAGCTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-mCherry-DMDEx23-eGFP was a gift from Ulrich Lächelt (Addgene plasmid # 211366 ; http://n2t.net/addgene:211366 ; RRID:Addgene_211366)

  • For your References section:

    mCherry on Top: A Positive Read-Out Cellular Platform for Screening DMD Exon Skipping Xenopeptide-PMO Conjugates. Lessl AL, Pohmerer J, Lin Y, Wilk U, Hohn M, Horterer E, Wagner E, Lachelt U. Bioconjug Chem. 2023 Dec 20;34(12):2263-2274. doi: 10.1021/acs.bioconjchem.3c00408. Epub 2023 Nov 22. 10.1021/acs.bioconjchem.3c00408 PubMed 37991502
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