pcDNA3-10His-StayGold
(Plasmid
#211362)
-
PurposeMammalian expression of the bright and photostable StayGold fluorescent protein. Contains a N-terminal 10xHis tag.
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 211362 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5381
- Total vector size (bp) 6071
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameStayGold
-
SpeciesSynthetic
-
Insert Size (bp)690
- Promoter CMV
-
Tag
/ Fusion Protein
- 10xHis-tag (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 Promoter
- 3′ sequencing primer SP6 Promoter (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byAtsushi Miyawaki: Hirano et al. (2022) Nat Biotechnol. doi: 10.1038/s41587-022-01278-2. PMID: 35468954
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.21203/rs.3.rs-2684100/v1 for ResearchSquare preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA3-10His-StayGold was a gift from Mohan Balasubramanian (Addgene plasmid # 211362 ; http://n2t.net/addgene:211362 ; RRID:Addgene_211362) -
For your References section:
A monomeric StayGold fluorescent protein. Ivorra-Molla E, Akhuli D, McAndrew MBL, Scott W, Kumar L, Palani S, Mishima M, Crow A, Balasubramanian MK. Nat Biotechnol. 2023 Dec 11. doi: 10.1038/s41587-023-02018-w. 10.1038/s41587-023-02018-w PubMed 38081970