pET-MCN-10His-mStayGold (E138D)
(Plasmid #211361)

• Purpose: Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.
• Depositing Lab: Mohan Balasubramanian
• Publication: Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.1038/s41587-023-02018-w.

Backbone

• Vector backbone: pET-MCN
• Backbone size w/o insert (bp): 5265
• Total vector size (bp): 5955
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: mStayGold
• Species: Synthetic
• Insert Size (bp): 690
• Mutation: E138D
• Promoter: T7
• Tag / Fusion Protein:
  • 10xHis-tag (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7 Promoter

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Atsushi Miyawaki: Hirano et al. (2022) Nat Biotechnol. doi: 10.1038/s41587-022-01278-2. PMID: 35468954

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.21203/rs.3.rs-2684100/v1 for ResearchSquare preprint.