pET-MCN-10His-StayGold
(Plasmid
#211360)
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PurposeBacterial expression of the bright and photostable StayGold fluorescent protein. Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 211360 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET-MCN
- Backbone size w/o insert (bp) 5265
- Total vector size (bp) 5955
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameStayGold
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SpeciesSynthetic
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Insert Size (bp)690
- Promoter T7
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Tag
/ Fusion Protein
- 10xHis-tag (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 Promoter (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byAtsushi Miyawaki: Hirano et al. (2022) Nat Biotechnol. doi: 10.1038/s41587-022-01278-2. PMID: 35468954
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.21203/rs.3.rs-2684100/v1 for ResearchSquare preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-MCN-10His-StayGold was a gift from Mohan Balasubramanian (Addgene plasmid # 211360 ; http://n2t.net/addgene:211360 ; RRID:Addgene_211360) -
For your References section:
A monomeric StayGold fluorescent protein. Ivorra-Molla E, Akhuli D, McAndrew MBL, Scott W, Kumar L, Palani S, Mishima M, Crow A, Balasubramanian MK. Nat Biotechnol. 2023 Dec 11. doi: 10.1038/s41587-023-02018-w. 10.1038/s41587-023-02018-w PubMed 38081970
Map uploaded by the depositor.
