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Addgene

m.5147-Right Puro DdCBE-G1397-C- T1413I-GFP
(Plasmid #211344)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211344 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression, TALEN
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Lower temperature is preferred
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    m.5147-Right Puro DdCBE-G1397-C- T1413I-GFP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3453
  • Promoter CMV
  • Tag / Fusion Protein
    • HA

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gacccaagctggctagcacc
  • 3′ sequencing primer cagaggacaaagacccctta
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    m.5147-Right Puro DdCBE-G1397-C- T1413I-GFP was a gift from Monkol Lek (Addgene plasmid # 211344 ; http://n2t.net/addgene:211344 ; RRID:Addgene_211344)
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