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AAVS-HsProm1s1-StayGold
(Plasmid #211333)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211333 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAVS1
  • Backbone manufacturer
    System Biosciences
  • Backbone size w/o insert (bp) 9001
  • Total vector size (bp) 12262
  • Vector type
    AAV
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Prominin 1
  • Alt name
    PROM1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3261
  • Entrez Gene
    PROM1 (a.k.a. AC133, CD133, CORD12, MCDR2, MSTP061, PROML1, RP41, STGD4)
  • Promoter EF1a
  • Tag / Fusion Protein
    • StayGold (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.11.08.566258 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAVS-HsProm1s1-StayGold was a gift from Luke Chao (Addgene plasmid # 211333 ; http://n2t.net/addgene:211333 ; RRID:Addgene_211333)

  • For your References section:

    Prominin 1 and Tweety Homology 1 both induce extracellular vesicle formation. Bell TA, Luce BE, Hakim P, Ananda VY, Dardari H, Nguyen TH, Monshizadeh A, Chao LH. Elife. 2024 Aug 13;13:e100061. doi: 10.7554/eLife.100061. 10.7554/eLife.100061 PubMed 39168849
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