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pLX304-HsTtyh1-TwinStrep-His
(Plasmid #211332)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211332 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLX304
  • Backbone manufacturer
    David Root
  • Backbone size w/o insert (bp) 7651
  • Total vector size (bp) 9202
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    30C/37C for plates, 30C for liquid growth
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tweety homology protein 1
  • Alt name
    Ttyh1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1551
  • Mutation
    W417R
  • Promoter CMV
  • Tag / Fusion Protein
    • TwinStrep, 10xHis (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    David Root (Addgene # 25890)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.11.08.566258 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLX304-HsTtyh1-TwinStrep-His was a gift from Luke Chao (Addgene plasmid # 211332 ; http://n2t.net/addgene:211332 ; RRID:Addgene_211332)

  • For your References section:

    Prominin 1 and Tweety Homology 1 both induce extracellular vesicle formation. Bell TA, Luce BE, Hakim P, Ananda VY, Dardari H, Nguyen TH, Monshizadeh A, Chao LH. Elife. 2024 Aug 13;13:e100061. doi: 10.7554/eLife.100061. 10.7554/eLife.100061 PubMed 39168849
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