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Addgene

pCMV-BID(D59E)
(Plasmid #21133)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21133 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pVMV-5a
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Flag-tagged Mutant mouse BID
  • Alt name
    PHL 183
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    585
  • GenBank ID
    EDK99651
  • Tag / Fusion Protein
    • Flage (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-BID(D59E) was a gift from Junying Yuan (Addgene plasmid # 21133 ; http://n2t.net/addgene:21133 ; RRID:Addgene_21133)

  • For your References section:

    Cleavage of BID by caspase 8 mediates the mitochondrial damage in the Fas pathway of apoptosis. Li H, Zhu H, Xu CJ, Yuan J. Cell. 1998 Aug 21. 94(4):491-501. 10.1016/S0092-8674(00)81590-1 PubMed 9727492
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