p2xFlag CMV2-YAP2-deltaC
(Plasmid
#21123)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 21123 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep2xFlag CMV2
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameYes-kinase associated protein
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Alt nameYAP2, YAP65
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Alt nameYAP2 isoform
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SpeciesH. sapiens (human)
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Insert Size (bp)1467
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Mutation5 Carboxyterminal amino acids -FLTWL are deleted
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GenBank IDNM_006106
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Entrez GeneYAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)
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Tag
/ Fusion Protein
- 2xFlag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer CMV (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p2xFlag CMV2-YAP2-deltaC was a gift from Marius Sudol (Addgene plasmid # 21123 ; http://n2t.net/addgene:21123 ; RRID:Addgene_21123) -
For your References section:
Nuclear localization and pro-apoptotic signaling of YAP2 require intact PDZ-binding motif. Tsutomu Oka & Marius Sudol. 10.1111/j.1365-2443.2009.01292.x PubMed 19371381