pcDNA3-miR29b(m4)
(Plasmid
#21122)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21122 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1(+)
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5428
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemiR-29b-1
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SpeciesH. sapiens (human)
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Insert Size (bp)423
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MutationG to C change at nucleotide 21 of the mature miR-29b sequence by site-directed mutagenesis
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Entrez GeneMIR29B1 (a.k.a. MIRN29B1, miR-29b, miRNA29B1, mir-29b-1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-miR29b(m4) was a gift from Joshua Mendell (Addgene plasmid # 21122 ; http://n2t.net/addgene:21122 ; RRID:Addgene_21122) -
For your References section:
A hexanucleotide element directs microRNA nuclear import. Hwang HW, Wentzel EA, Mendell JT. Science. 2007 Jan 5. 315(5808):97-100. 10.1126/science.1136235 PubMed 17204650