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Addgene

pcDNA3-miR29b
(Plasmid #21121)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21121 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    miR-29b-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    423
  • Entrez Gene
    MIR29B1 (a.k.a. MIRN29B1, miR-29b, miRNA29B1, mir-29b-1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-miR29b was a gift from Joshua Mendell (Addgene plasmid # 21121 ; http://n2t.net/addgene:21121 ; RRID:Addgene_21121)

  • For your References section:

    A hexanucleotide element directs microRNA nuclear import. Hwang HW, Wentzel EA, Mendell JT. Science. 2007 Jan 5. 315(5808):97-100. 10.1126/science.1136235 PubMed 17204650
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