pGL3con-miR143Per
(Plasmid #21118)

• Depositing Lab: Joshua Mendell
• Publication: Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 9. ():.

Backbone

• Vector backbone: pGL3-control
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5256
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hsa-miR-143 sensor
• Species: Sequences containing two perfect complementary sites for hsa-miR-143
• Insert Size (bp): 56

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (destroyed during cloning)
• 3′ cloning site: XbaI (destroyed during cloning)
• 5′ sequencing primer: GTACCGAAAGGTCTTACCG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry